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Longitudinal studies show that gastrointestinal symptoms such as constipation may precede motor manifestations by up to two decades, supporting the involvement of gutbrain axis [120]

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Louis, MO), The lysate was clarified by centrifugation at 15,000 g for 15 minutes, protein concentrations were determined using the BCA total protein assay, and then appropriate volumes of the lysates were diluted with 2x reducing Laemmli buffer and heated for ten minutes at 95 C