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MRT techniques include spindle transfer (ST), pronuclear transfer (PNT), and polar body transfer (PBT) [383]

In our outpatient clinic, high-dose glutathione infusions with other immunomodulators such as mistletoe, thymus, enzymes or cytokines are successfully used, partly supported by other antioxidants such as selenium and vitamins

The tissue was disrupted using tissue homogenizer Bio-Gen PRO200 homogenizer (PRO Scientific, Oxford, CT, USA), in 1000 l of TRIzol (Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA) and the isolation itself was done using a TRIzol-based reagent method according to the manufacturers instructions

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The Stroke Prevention Trial in Sickle Cell Anemia (STOP), the follow-up STOP2, and the Silent Cerebral Infarct Transfusion (SIT) trial demonstrated that chronic transfusion reduces stroke risks (Ware et al., 2016)