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Determination of SOD activity The total activity of SOD was measured in the supernatant of heart tissue homogenate by the Cayman Chemical SOD Assay kit (USA) which utilizes a tetrazolium salt to detect the generated superoxide radicals by measuring the absorbance at 420 nm

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doi: 10.1128/MCB.21.4.1249-1259.2001

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Findings are often non-specific and appear only in late stages (e.g., pneumoperitoneum from perforation), making it an unreliable tool for exclusion[108, 112]

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