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The decrease of serum levels of LH and testosterone indicates the hypothalamic-pituitary-gonadal axis dysfunction, which is closely associated with PACS-induced ED (Leng T

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The remaining studies in patients with epilepsy also demonstrated high serum and CSF levels of multiple proinflammatory cytokines, including IL-6, IL-17, TNF-, transforming growth factor (TGF)-, and interferon (IFN)- (149153)

Cells were fixed with 4% PFA in PBS at room temperature for 15 min, permeabilized with 1% saponin and stained by rabbit anti-ACP1 antibody (LifeSpan Bioscience) followed by Alexa 488-conjugated goat anti-rabbit IgG antibody (Invitrogen)

All reagents were diluted in PBS2% BSA

We were therefore surprised to find that GSTP1 knockdown did not significantly influence cell growth rate or alter cell cycle progression in A2780 cells, although similar findings have been reported in a series of ovarian cancer cell lines made resistant to cisplatin, carboplatin and paclitaxel (Li et al, 2004), suggesting limitations of the use of immortalised cancer cell line models