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GSH and GSSG determination To assess the influence of FLC and MCFG on glutathione intracellular contents, each strain was cultured in YNB medium at 28C, for 24 h, using an inoculum size of approximately 10 7 cells/ml to recover adequate amount of cytosolic material for glutathione analysis

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This overlap between gastrointestinal improvement and lifestyle changes appeared frequently throughout the dataset

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An example of this occurred in 2014 when two young men died after ingesting powdered caffeine containing 25 coffee cups worth of caffeine per teaspoon