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We also assessed the neuronal pathological changes in acquired digital images saved as uncompressed 24-bit RGB TIFF files in the software program AnalySIS (Olympus Soft Imaging System GmbH, Munster, Germany), performing quantitative analysis of neuronal damage in the karyopyknotic areas

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After 12 treatment sessions, patients were assessed for pain intensity, knee flexion range of motion, a timed up-and-go test, a 6-min walk test, and knee functionality compared to pre-treatment baseline measurements