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In cases in which values were lower than (LOQ) of the assay, a value of LOQ/square root of 2 was assigned to the sample (Finkelstein and Verma 2001)

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For example, while it is possible to find several efficient established protocols to successfully isolate protoplasts from model plants and crops, it is not feasible to isolate enough viable protoplasts from all organs and tissues of all plants due to species specificity constraints ( in situ structural studies of vascular plant cells, yet protoplasts are isolated cells outside of the context of tissue environment, which needs to be considered with respect to cell physiology and impact for the underlying cellular architecture