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In rodent models, the peptide activated VEGF-driven angiogenesis, nitric-oxide signaling, and fibroblast migration (Park, 2025)

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However, this method is not perfect as centrifugation can result in either the undesired loss of IGF-1 from the sample or retention of residual IGFBPs in the supernatant [113]

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Untreated cells in 2% or 10% FBS DMEM for 3 days were used as negative controls
