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Expert Review of Dermatology

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American Journal of Reproductive Immunology, 2012

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2013;8(11):e79823

Material and methods Tissue culture, cell, and constructs The human melanoma cell line SK-MEL 28 was obtained from American Tissue Culture Collection (ATCC, Manassas, VA) and maintained in Dulbeccos modified Eagles medium (DMEM) (Invitrogen Life Technologies, Burlington, ON) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (FBS) (Invitrogen Life Technologies, Burlington, ON), 2 mM glutamine and 20 mM Hepes