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the lysate was clarified by centrifugation at 15,000 g for 15 minutes, protein concentrations were determined using the BCA total protein assay, and then appropriate volumes of the lysates were diluted with 2x reducing Laemmli buffer and heated for ten minutes at 95 C

Marso SP, Daniels GH, Brown-Frandsen K, et al

doi: 10.1152/ajpcell.00210.2022 [DOI] [PMC free article] [PubMed] [Google Scholar] 62.Gilman AG

Podgrska, A., Pucion-Jakubik, A., Markiewicz-ukowska, R., Gromkowska-Kpka, K.J

BPC-157 is a repair assist for joint pain, not a cure for arthritis

Additionally, this review discussed the potential impact of biomaterials and AI in medical research on understanding and treating radiation-induced skin fibrosis