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Similar to ncr-1(0) mutants, ncr-2(0) mutants showed higher levels of cholesteryl ester compared with the WT (Fig 2C), supporting that NCR-2 also aids in the hydrolysis or inhibits the formation of cholesteryl esters

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Either that or you have to use 12v car batteries as the input power, but in a lab this is cumbersome and space consuming

This eliminates human error and ensures you are always drawing the correct volume

[PubMed ID: 1355577]