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The cell membrane that functions as a physical barrier, limits the intracellular delivery of therapeutics, especially peptide, protein, nucleic acid, and nanocarrier drugs to their molecular targets

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Electron microscopy reveals that the inner diameter of GSDMD-N pores ranges from 10 to 15 nm, allowing the passage of certain pro-inflammatory cytokines such as IL-1 and IL-18, thereby triggering pyroptosis ( 2.1.2.2 NOD-like receptor thermal protein domain associated protein 3 Inflammasome The NLRP3 inflammasome is a substantial multiprotein complex, approximately 700,000 Da in molecular weight, consisting of NLRP3, the adaptor protein ASC, and the effector protein caspase-1

doi: 10.1016/j.molcel.2014.03.030

Temperature was kept at 37 C using a rectal thermometer-controlled heatpad (TCAT-2LV, Physitemp, USA)