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USA 119 , e2118646119 (2022)

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Though the typical clinical appearance is enough to diagnose the case, other differential diagnoses must to be ruled out, such as venereal diseases by serological tests

ECG Recording ECGs were recorded continuously in deeply anesthetized rats for all three main leads, by positioning stainless steel electrodes on all four limbs using an ECG monitor with a 2090 programmer (Medtronic, Minneapolis, MN, USA) connected to a Waverunner LT342 digital oscilloscope (LeCroy, Chestnut Ridge, NY, USA) at 15 min, 24 h or 48 h ligation-time