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Cells can be lysed directly using denaturants such as urea or guanidine-HCl

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Manifestation of Porphyria In patients with porphyria, specific genetic or acquired deficiencies limit the flow of heme precursors through the cascade of steps needed to form hemoglobin

Zevin and Benowitz, 1999

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Collectively, these considerations explain why -HB has become the principal focus of research, as it is thought to account for the majority of the therapeutic effects associated with KD