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Co-immunoprecipitation (co-IP) After treatment, the cells were lysed in an ice-cold co-IP buffer containing 20 mM Tris-HCl (pH 8.0), 100 mM NaCl, 1 mM EDTA, and 0.5% NP-40, supplemented with a protease inhibitor cocktail (Roche, Cat

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The oxidative stress induced by these neurotoxicants activates/inhibits signaling pathways leading to augmented/diminished activity of enzymes that promote the accumulation of toxic materials in neural cells such as damaged/aberrant proteins, A in AD or syn in PD and oxidative byproducts, or the oxidation of DNA that can alter genetic or epigenetic regulation

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The higher the activity score, the higher the enzyme activity of the CYP2D6