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The modifications (amino acid substitutions at positions 2, 8, 15, and 27) improve metabolic stability without altering receptor binding affinity
Shinoda, G

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Due to the impermeability of the mitochondrial inner membrane to fatty acyl-CoA thioesters, the specialized transporting system has evolved to transport fatty acids across mitochondrial membranes

Serine 615 phosphorylation enhances eNOS activity, working cooperatively with Ser1177 to enhance the binding affinity of the Ca 2 -CaM complex to eNOS, a critical step for eNOS activation, which ensures a robust response to Ca 2 signals (Tran et al., 2009