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These circumstances lead to differences between DDA or MRM analyses (e.g., RLM (1D) compared to 3A4 (1D) in Table 3), leading to more found modified sites with RLM incubations than with Supersomes, even though the latter were less complex incubations

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[DOI] [PMC free article] [PubMed] [Google Scholar] [115].Basit F, van Oppen LM, Schockel L, et al

Sample preparation for LC-MS/MS typically involves steps such as protein precipitation or liquid-liquid extraction to isolate the target vitamins from the complex biological matrix before chromatographic separation and subsequent mass spectrometric detection (Konieczna et al., 2016

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