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Researchers typically aim for a concentration that allows for precise measurement of the 20mg total peptide content
On the other hand, small concentrations of PCTX do not increase microtubule polymerization, but act as microtubule-stabilizing agents, blocking depolymerization, thus the anaphase cannot be achieved and apoptosis mechanisms are activated (Tamburin et al., 2019)
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The purified recombinant protein was dissolved in carbonate buffer (pH 9.6) and coated on polystyrene 96-well microtiter plates (Corning, Inc., NY, USA) overnight at 4 C at a concentration of 50 g/mL, 100 L per well
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Albert GC, Cook CM, Prato FS, Thomas AW
