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microRNA miR-144 modulates oxidative stress tolerance and associates with anemia severity in sickle cell disease

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Blots were first incubated with 5% BSA and 0.1% Tween in Tris-buffered saline (TBS) for 1 h at room temperature, and then with either polyclonal rabbit primary antibody against Orai1 (1:1,000, rabbit Sigma-Aldrich, O8264), STIM2 (1:2,000, rabbit Sigma-Aldrich, S8572) or monoclonal mouse actin (1:100, mouse Sigma-Aldrich, A2066) at 4C overnight

doi: 10.1016/j.soard.2011.04.036

In addition, they demonstrated that quercetin also prevents aluminum-induced translocation of cytochrome c , upregulates Bcl-2, down-regulates Bax , p53 , and caspase-3 activation, and reduces DNA fragmentation [109]