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Click reaction mixture was prepared by sequential addition of 1 volume 10 mM azide-PEG3-biotin (Sigma, 762024), 2 volumes 50 mM CuSO 4 (Sigma, C8027), 2 volumes 50 mM TCEP (Sigma, 68957) and 1 volume 10 mM TBTA (Sigma, 678937)

[DOI] [PubMed] [Google Scholar] 211.Stanciu G.D., Luca A., Rusu R.N., Bild V., Beschea Chiriac S.I., Solcan C., Bild W., Ababei D.C

It helps to defend the the body against damage such as cigarette smoking, exposure to radiation and alcohol
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Pinto-Cortez, H., et al., Epidemiology of Nonalcoholic Fatty Liver Disease and Nonalcoholic Steatohepatitis: Implications for Liver Transplantation
Cell lysates were boiled for 15 min and protein concentration was determined by the Lowry method