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Furthermore, as these cells undergo successive passages in culture, the expression of these critical activation genes shows a progressive decline 13,14

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In recent studies using BRET (Bioluminescence Resonance Energy Transfert) or FRET (Fluorescence Resonance Energy Transfer) techniques, a large panel of opioid ligands were shown to have a different ability to both activate G proteins and recruit arrestins at MOR and DOR (Mcpherson et al., 2010