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Behavioral assessments (open-field, self-grooming, social interactions) were followed by biochemical and molecular analyses in the cerebellum, where oxidative stress markers (MDA, GSH, SOD, CAT, and GPx) were quantified spectrophotometrically, and Nrf2, Bax, Bcl-2, and Caspase-3 mRNA levels were measured by q RT-PCR
Conclusion In conclusion, the present study demonstrates that children with ASD exhibit distinct but subtle plasma metabolomic features , particularly involving branched-chain amino acid metabolism, methyl-donor pathways, and diet-responsive bioactive compounds
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doi: 10.3390/biomedicines11102814 34 YennemadiASJordanNDiongSKeaneJLeischingG
