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To determine whether this ARE is functional to mediate NRF2-dependent upregulation of CBS gene expression, we cloned CBS promoter fragment from 1000 to +200 bp and mutant fragment with deletion of +77 to +87 bp into a pGL3-basic luciferase reporter vector, which were designated as pGL3-phCBS and pGl3-phCBS-ARE, respectively

Zinc deficiency can cause serious disturbances in the functioning of GABA receptors, leading to increased anxiety and other neurological symptoms

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