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The membrane was blocked with 3% non-fat milk (BioRad, Hercules, CA, United States) at room temperature for 1.5 h, washed, and incubated with primary antibodies (Supplementary Table 3) at 4C overnight on a rotating shaker

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38,5658 In contrast, animals on a normal diet experienced significant centrilobular necrosis with only limited LPO and no death within 24 h (Fig

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Evaluating Overall Vitality Overall vitality is a broad term that encompasses your dog's general state of health and well-being