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Papain (50 g/reaction) was activated in the assay buffer (50 mM Tris-HCl pH 7.6, containing 2 mM cysteine and 0.1 mM EDTA, pH 6.5) for 30 minutes at 40C, and the papain substrate was diluted in DMSO

Mitochondrial glutathione redox and energy producing function during liver ischemia and reperfusion

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IR injury also triggered substantial elevations in apoptotic markers (271% increase in Bax (Bcl2-associated X protein) and 65.9% decrease in BCL2 (B-cell lymphoma 2), and inflammatory mediators (285.7% increase in NFB (nuclear factor-kappa B)

Mitochondrial longevity pathways

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