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Phenolic compounds in Brassica vegetables

Cells were trypsinised, washed and pelleted at 1000 x g for 5 min at 4 C and subsequently resuspended in SET buffer (0.25 M sucrose, 1 mM EDTA, 20 mM Tris-HCL, pH 7.5)

FBXO31 sensitizes cancer stem cells-like cells to cisplatin by promoting ferroptosis and facilitating proteasomal degradation of GPX4 in cholangiocarcinoma

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Then, phalloidin-FITC (1 M) was incubated with the cells for 30 min at 37 C to visualize actin filaments