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This product is supplied as a pre-reconstituted research compound at a concentration of 200mg/mL in a 30mL vial, intended strictly for laboratory and in vitro research purposes

10.1371/journal.pone.0127866 110 PatriotaL

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(The supernatant can be stored at -80 for 10 days.) Cell sample Harvesting cell should not less than 106,then wash with PBS for twice (mix cell with PBS centrifuge at 600g for 10 minutes), mix precipitated cell with the volume of PBS for 3 times

Multi-generational impacts of exposure to antidepressant fluoxetine on behaviour, reproduction, and morphology of freshwater snail Physa acuta