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UPS cells maintain glutamine anaplerosis Glutamine catabolism plays a critical role in sustaining TCA cycle activity by initial conversion to glutamate for -ketoglutarate (-KG) generation, an important intracellular carbon source 17,39

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The reaction was stopped with 0.16 M H 2 SO 4 (for the IL-6 ELISA kit), and the samples were analyzed at 405, 450, 450 and 420 nm for IL-8, IL-6, IL-1 and PGE2, respectively, in a Varioskan Flash Reader (Thermo Fisher Scientific)

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[DOI] [PMC free article] [PubMed] [Google Scholar] 49.Paris S., Debeaupuis J.P., Crameri R., Carey M., Charles F., Prevost M.C., Schmitt C., Philippe B., Latg J.P