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After an incubation for 30 min at 25 C with gentle rocking, beads were pulled down with the magnetic rack, with supernatant collected as flowthrough

1 Introduction Low back pain (LBP) and intervertebral disc degeneration (IDD) are widespread issues, affecting about two-thirds of adults and impacting approximately 568 million people globally (Hurwitz et al., 2018)

After euthanasia, the knee joints were surgically exposed, and the femoral and tibial condyles were examined for gross morphological changes

The decrease in absorbance was measured by spectrophotometric reading at 340 nm at 25 C after the addition of H 2 O 2

doi: 10.1016/j.ajme.2017.09.001 166 GopinathKSudhandiranG

As an emulsifier, it helps stabilize oil-in-water emulsions