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The supernatant was mixed with SDS sample buffer [50 mM Tris-HCl pH 6.8, 2% (w/v) SDS, 5% (w/v) glycerol, 0.02% (w/v) bromophenol blue, and 200 mM DTT] and heated at 70 C for 20 min

(2012) evaluated 29 children with ASD, 14 who were administered NAC and 15 placebo, using the Dosage Record and Treatment Emergent Symptom Scale (DOTES), Clinical Global Rating Scale (CGRS), Aberrant Behavior Checklist (ABC), Social Responsiveness Scale (SRS), and the Sensory Profile Questionnaire (SPQ)

It is a well-established principle in chemistry called the Arrhenius equation, and it applies directly to peptides in solution

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Specifically, Mfn1 deficiency leads to marked mitochondrial fragmentation, while Mfn2 deficiency results in a higher proportion of mitochondria with spherical or ovoid shapes [45]