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Description

Equal amounts of protein (30 g) were loaded onto 10% SDS-PAGE gel, then electrophoresed and transferred to 0.2 m PVDF membrane (Millipore, Darmstadt, Germany), which were blocked with 5% BSA for 2 h and incubated with the following primary antibodies overnight at 4C: against COX-2 (1:1000), iNOS (1:2000), p-NF-B p65 (1:1000), NF-B P65 (1:1000), p-AKT (1:2000), AKT (1:2000), p-P38 (1:2000), P38 (1:1000), p-ERK1/2 (1:2000), ERK1/2 (1:2000), p-JNK (1:2000), JNK (1:2000), and -actin (1:2000)

Seems to be very positively impacted by using estrogen hormone therapy

[2] It usually lasts only a few days, and while it often resolves on its own, it can still be uncomfortable and disruptive if not addressed

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