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Cytosolic and nuclear NAD + are often considered a shared pool as NAD + diffuses through nuclear pores 58 , and importantly, the concentrations of NAD + in the cytosol, nucleus, and mitochondria mirror the K m of NAD + -consuming enzymes localized to the corresponding compartment, hence these enzymes are sensitive to compartmental fluctuations in NAD + levels 77

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Incidental exposure to ethanol (e.g., mouthwash, hand sanitizer) can yield positive urinary EtG and EtS results, so an appropriate analytical cutt-off and combining results from different biomarkers is essential (128)