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It consists of 521 amino acids, has a molecular mass of 56.416 kDa, and is encoded on chromosome 2q37.3

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Experimental Incubation of Cells To study the consequences of a treatment of astrocytes with IAA or IA, the cells were washed with 1 mL of prewarmed (37C) incubation buffer (IB

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Additionally, similar to strategies employed in bacterial systems, attempts were made to introduce mutations into the tRNAs EF binding region to further enhance efficiency ( 3.2.5 Customizing tRNA for UAA incorporation at multiple sites Achieving multiple-site incorporation of UAA relies crucially on enhancing suppression efficiency and establishing new orthogonality