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Next, cells were washed with PBS, counterstained with 4-6-diamidino-2-phenylindole (Invitrogen), and mounted with Vectashield Hardset mounting media (Vector Laboratories)

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The modified carriers and immobilized lipase were investigated using X ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), X-ray photoelectron spectroscopy (XPS), transmission electron microscopy (TEM), atomic force microscopy (AFM) and confocal laser scanning microscopy (CLSM)