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For targets with no existing fluorophore conjugated antibody the following primary antibodies were used: anti NRF2 polyclonal (1:200, Thermofisher, PA5-27882)

The damage caused by oxidation depends on the defects of the enzymes involved in the redox signaling pathways (Tan et al., 2018)

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The day of the analysis, the oocytes were placed on a glass slide with a 1 L drop of SlowFade and 5 g/mL Hoechst 33,342 under a coverslip

It works by mimicking the hormone glucagon-like peptide-1 to regulate blood sugar, slow gastric emptying, and reduce appetite