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Materials and Methods GSH/GSSG-Glo Assay (Cat.# V6611) CellTox Green Cytotoxicity Assay (Cat.# G8741) menadione (Sigma, Cat.# M5625) l-Buthionine Sulfoximine (Sigma, Cat.# B2515) digitonin (Sigma, Cat.# D141) HEK-293 cells K-562 cells Note: Cell lines were authenticated using STR genotyping ( GenePrint 10 System, Cat.# B9510)

Another observational study from India shows that edaravone infusion does not stop or significantly slow progression of ALS from baseline but is safe (34)

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Braymer JJ, Freibert SA, Rakwalska-Bange M, Lill R

Given the low density of mitochondria in the somatodendritic region of SNpc DA neurons, the transportation of mitochondria to axons may be necessary, which presents a challenge for mitochondrial trafficking [35]

At least 2 hours fasted before, 30 minutes after