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For a summary of the worker findings, see Table 1

doi: 10.1007/s00213-011-2619-3

After 1618 hours of incubation, cells were harvested by centrifugation and resuspended in freshly prepared lysis buffer (25 mM HEPES, 500 mM NaCl, 20 mM cysteine, pH 7.4)
Studies have revealed the presence of IL17secreting cells across all AA types, with a higher prevalence observed in multiple patchy alopecia and a lower occurrence in AT, predominantly localized in the periphery of the hair follicle [74]

doi: 10.1016/j.cytogfr.2023.06.002 [DOI] [PubMed] [Google Scholar] 85

Thirty years of nrf2: advances and therapeutic challenges