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Trx-S2 can be reduced to Trx-(SH)2 by the exchange reaction of -SS- and -SH under the action of thioredoxin reductase (TrxR) and nicotinamide adenine dinucleotide phosphate (NADPH)

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Abbreviations MALDI-TOF MS: Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry Hb: Hemoglobin GPX4: Glutathione peroxidase 4 AA: Arachidonic acid PUFA: Sphinganine, polyunsaturated fatty acid TCA: Tricarboxylic acid cycle LDH: Lumbar disc herniation RBCs: Red blood cells HNPCs: Human primary nucleus pulposus cells DFO: Deferoxamine mesylate salt FAC: Ferric ammonium citrate PMSF: Phenylmethylsulfonyl fluoride HMDB: Human metabolome database HO-1: Heme oxygenase-1 OD: Optical density PCA: Principal component analysis ROC: Receiver operating curve (ROC) ROS: Reactive oxygen species (ROS) References Ala-Kokko L

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