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Removal of antibiotic resistance genes in two tertiary level municipal wastewater treatment plants

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Step 3: Inject with Minimal Discomfort To make the injection as painless as possible, start by cleaning the chosen spot with an alcohol wipe and letting it air-dry completely

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Total RNA isolation and mRNA sequencing sample preparation The total RNA isolation and sequencing library preparation from IR-induced senescent and proliferating IMR90 cells was performed as follows: total RNA was isolated using Qiazol Lysis Reagent (Qiagen) and purified with the miRNeasy kit (Qiagen)