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Altered expression of KCN family members, possibly compensatory, and non-KCN factors may provide quantities of K + sufficient for preservation of cell membrane integrity and K + /H + exchange on inner membranes to release bound H + for pH reversal and invasion

doi: 10.3389/fnins.2022.901360 Received 21 March 2022 Accepted 23 May 2022 Published 10 June 2022 Volume 16 - 2022 Edited by Yongjun Sun, Hebei University of Science and Technology, China Reviewed by Chunhua Chen, Peking University, China

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While roGFP2 WT was completely oxidized at even very low PfGrx C32S/C88S (SSG) concentrations, 1 M PfGrx C32S/C88S (SSG) glutathionylated approximately 50% of 1 M roGFP2 C151S (SH) suggesting that the redox couples roGFP2 C151S (SSG)/roGFP2 C151S (SH) and PfGrx C32S/C88S (SSG)/PfGrx C32S/C88S (SH) have very similar redox potentials

Protein carbonyls, an index of oxidative protein damage, were detected using the DNPH reaction followed by Western blotting analysis of insoluble protein fractions [28]