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10.3390/pharmaceutics13111745 CrossRef Google Scholar View reference in article 48 KameiN.Takeda-MorishitaM

Eluted protein was further purified by HiTrap Heparin HP affinity column (Cytiva, 17040703) with a linear gradient of Low-Salt Wash Buffer (50 mM HEPES-NaOH pH 7.4, 50 mM NaCl, 2 mM EDTA, 10% Glycerol, and 2 mM DTT) and High-Salt Wash Buffer (50 mM HEPES-NaOH pH 7.4, 1 M NaCl, 2 mM EDTA, 10% Glycerol, and 2 mM DTT) to remove RNA contamination

Fine-tune your treatment plan for optimal results ALDAs practitioners remain readily available to address your questions and ensure your confidence throughout the process

Hulse EJ et al (2019): Organophosphorus nerve agent poisoning: managing the poisoned patient

Once reconstituted, the solution should be kept refrigerated and is typically stable for up to 2 months under proper storage conditions

Protein expression We used the Bac-to-Bac baculovirus system (Thermo Fisher) in Spodoptera frugiperda ( sf9 ) cells for expression