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The cell suspension was centrifuged at 500 g for 5 min at 4C and the cell pellet was incubated with ACK lysing buffer (Thermo Fisher Scientific, A1049201) for 5 minutes to lyse the red cells

Theeffect of Sambucol, a black elderberry-based, natural product, on theproduction of human cytokines: I

[DOI] [PMC free article] [PubMed] [Google Scholar] Tsukakoshi K., Yoshida W., Kobayashi M., Kobayashi N., Kim J., Kaku T., et al

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Glutamine may act as an anaplerotic substrate in neurons, entering the TCA cycle as ketoglutarate (Figure 3), thus being able to replenish the levels of TCA cycle intermediates