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Dietary emulsifiers impact the mouse gut microbiota promoting colitis and metabolic syndrome
At the indicated times, cells were lysed on ice for 10 min using pre-cooled TBS containing 0.02% saponin
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Given that the regulation of the intracellular cytoskeleton is dependent on the presence of ATP to stimulate the contraction of actin-myosin complexes, a reduction in ATP concentration caused by NSAIDs was found to impair TJs and reduce cellular polarity (Knig et al
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Importantly, this conclusion is independent of any problems with, for example, antibodies leaving their epitopes
