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The 2013 Journal of Neurochemistry paper by McCoy et al

I was seriously deficient in vitamin B12 something Id never heard of

The day before the assay, frozen aliquots of the above-mentioned BHK cell lines were thawed, washed once in PBS (Gibco, #14190-094) and seeded in 40 L medium in a white 384-well culture plate (PerkinElmer, #6007688) at a cell density of 4000 cells/well and incubated in plastic bags over night at 37 C and 5% CO 2

A penetration enhancer which could be solid, semisolid or liquid

However, it is always safer to use such medicines under the supervision of a doctor

Advances in Biochemistry in Health and Disease 22