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Plates were incubated at RT for 60 min before measuring the fluorescence intensity at 620 nm and 650 nm by an EnVision multilabel plate reader (PerkinElmer, Boston, USA)

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Large-scale purification was conducted and the peptide 20GIPR/GLP-1RG s complexes were collected by SEC for cryo-EM studies, with all components of the complex identified in SDS-PAGE of the SEC peak (Supplementary Fig

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