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Finally, human tissue samples, such as those from adipose tissue, skeletal muscle, and pancreatic islets, obtained from diabetic patients or healthy volunteers, present a direct window into SLC7 transporter activity in human metabolism

10.1016/j.coemr.2021.03.021 45 CasagrandeM.ForteG.FavieriF.CorboI

The fix was removed by one rinse with PBS

1B), which matched well with the reported values 21

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The medium was then removed, and serial dilutions of the protein samples (200 L/well) were added to the cells (unfused WT albumin, GLP-1 WT, and GLP-1 QMP: 0.086250 nM