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The cells were then washed three times with PBS, drained and mounted

These molecules activate immune-sensing pathways in microglia, including TLR4, P2X7, and cGASSTING, leading to the robust release of pro-inflammatory cytokines such as interleukin-1 (IL-1) and tumor necrosis factor- (TNF-) (Ayton et al., 2013)
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Adequate protein intake provides amino acids necessary for collagen synthesis