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e ) Volcano plot of differentially expressed analytes between patients with vs without brain fog

Fluorescence images were acquired with a fluorescence microscope (Thermo Fisher) or a confocal laser scanning microscope (Fluoviewver.3.1, Olympus)

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We discuss how copper also plays a key role in proteostasis and interacts either directly or indirectly with many of the key aggregate-prone proteins implicated in ALS, such as TDP-43, C9ORF72, SOD1 and FUS as well as the effect of their aggregation on copper homeostasis

The mechanistic target of rapamycin (mTOR) signaling is a key modulator of anabolic and catabolic reactions 34 , which in turn could affect ROS balance