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Measurement of glutathione peroxidase activity in mouse BALF Selenium-dependent glutathione peroxidase activity in mouse BALF was measured by monitoring fluorescence at 460 nm with an excitation wavelength of 355 nm in a Berthold plate reader (Tristar LB941, Bad Wildbad, Germany) 69

GCLM polyclonal (1:200, Proteintech, 14241-1-AP), GLRX polyclonal (1:200, Proteintech, 15804-1-AP)

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These K ATP channel openers induced analgesic effects by activating specific signaling pathways, such as the growth arrest-specific 6 (Gas6)/Axl/suppressor of cytokine signaling 3 (SOCS3) pathway in microglia, which helps attenuate neuroinflammation and postoperative pain (Zhu et al., 2015

AJ and HR performed the experiments
