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Prior to modification, M pro was treated with 50 mM TCEP for 30 minutes to ensure all cysteines were in their reduced form and then TCEP removed by multiple washes through an Amicon 10 kDa cutoff filter with pH 6.8 incubation buffer (50 mM tris-HCL, 2 mM EDTA and 100 mM NaCl)
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